- 1H7 (See other available formats)
- Regulatory Status
- Other Names
- CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
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C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.Product Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Host Species
- Recombinant LIP
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ChIP, IP, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Recognizes three mouse isoforms: C/EBP ß, LAP, LIP. Does not cross-react with human.
(PubMed link indicates BioLegend citation)
- Su, et al. 2003. J. Biol. Chem. 278:51150. (ChIP)
- Blidner AG, et al. 2015. J Immunol. 194:3452. PubMed
- Product Citations
AB_315675 (BioLegend Cat. No. 606202)
- C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively
- Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
- CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
- Phosphorylation, Ubiquitination
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.
- Growth hormone
- Gene ID
- 12608 View all products for this Gene ID 25301 View all products for this Gene ID
- View information about C/EBP beta on UniProt.org
|Purified anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP)||1H7||WB, ChIP, IP, ICC|
|Biotin anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP)||1H7||WB|
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