- TU27/Tubulin (See other available formats)
- Regulatory Status
- Other Names
- Tubulin, beta 2A
Covance Catalog# MMS-410P
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Tubulin is ubiquitously expressed and is the major constituent of microtubules, which are composed of alpha and beta tubulin heterodimers. There are five distinct forms of tubulin: alpha, beta, gamma, delta, and epsilon. Beta tubulin has multiple isoforms that are expressed in mammalian tissues and localize to different places in the cell.Product Details
- Verified Reactivity
- Bovine, Rat, Chinese Hamster, Sea Urchin, Chlamydamonas, Drosophila
- Antibody Type
- Host Species
- This antibody was generated against taxol-assembled microtubules.
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
WB, ICC, IHC
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1 µg/mL (1:1000)
• IF: 1,000
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
This antibody is effective in immunoblotting and immunostaining.
*Predicted MW = 55 kD
The TU27 clone is broadly reactive to b-tubulin subtypes and binds outside of the C-terminal isotype domains, but does not cross react with alpha-tubulin by immunoblotting. It recognizes a highly conserved epitope and reacts with ß-tubulin found in vertebrate as well as invertebrate tissues.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL in Drosophila and 1.0 µg/mL in other species.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2565030 (BioLegend Cat. No. 903401)
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Gene ID
- 533307 View all products for this Gene ID
- View information about beta-Tubulin on UniProt.org
|Purified anti-β-Tubulin||TU27/Tubulin||WB, ICC, IHC|
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