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PerCP/Cyanine5.5 anti-mouse TCR Vγ1.1/Cr4 Antibody

Pricing & Availability
Clone
2.11 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vgamma 1.1, TCR Vγ1 (Tonegawa nomenclature), TCR Cr4, TRGV1, GV5S1
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
2dot11_PerCPCyanine55_TCRVgamma1
C57BL/6 mouse splenocytes were stained with TCR ?/d FITC and TCR V?1.1 (clone 2.11) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • 2dot11_PerCPCyanine55_TCRVgamma1
    C57BL/6 mouse splenocytes were stained with TCR ?/d FITC and TCR V?1.1 (clone 2.11) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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141111 25 µg 128€
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141112 100 µg 282€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. TCR Vγ1.1 (Garman nomenclature) is also called TCR Vγ1 (Tonegawa nomenclature). The Vγ1 gene almost exclusively rearranges to the Jγ4-Cγ4 gene. Vγ1- Jγ4-Cγ4 expressing cells constitute a major population of γ/δ T cells in the thymus and peripheral lymphoid organs in adult mice, but they are only composed of a minor population of γ/δ T cells during fetal and early postnatal life. Vγ1 T cell development can happen in thymus-dependent and thymus-independent manners. Further studies have shown that the antibody 2.11 recognizes an epitope located in the Cr4 domain.

Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
T3.13.1 T-cell hybridoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Pereira P, et al. 1995. J. Exp. Med. 182:1921. (IP)
  2. Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)
  3. Pereira P, et al. 1996. Intl. Immunol. 8:83. (FC)
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
RRID
AB_2750516 (BioLegend Cat. No. 141111)
AB_2750517 (BioLegend Cat. No. 141112)

Antigen Details

Structure
Ig superfamily
Distribution

Subset of γ/δ T-cells

Function
Antigen recognition
Ligand/Receptor
Antigen
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Pereira P, et al. 1995. J. Exp. Med. 182:1921.
2. Grigoriadou K, et al. 2002. J. Immunol. 169:3736.

Gene ID
110067 View all products for this Gene ID
UniProt
View information about TCR Vgamma1.1/Cr4 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09.20.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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