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PerCP/Cyanine5.5 anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-16B4 (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1
Isotype
Mouse IgG1, κ
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Product Citations
publications
TW7-16B4_PerCPCy5.5_LAP_Antibody_FC_1_092412
C57BL/6 mouse splenocytes were stimulated with anti-mouse CD3, CD28, and recombinant mouse IL-2 for 48-hours, then surface stained with CD4 FITC and LAP (TGF-β1) (clone TW7-16B4) PerCP/Cyanine5.5 (top) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (bottom). This was followed by intracellular staining with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_PerCPCy5.5_LAP_Antibody_FC_1_092412
    C57BL/6 mouse splenocytes were stimulated with anti-mouse CD3, CD28, and recombinant mouse IL-2 for 48-hours, then surface stained with CD4 FITC and LAP (TGF-β1) (clone TW7-16B4) PerCP/Cyanine5.5 (top) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (bottom). This was followed by intracellular staining with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_PerCPCy5.5_LAP_Antibody_FC_2_092412
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
141409 25 µg 90€
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141410 100 µg 278€
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Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1-transduced P3U1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1. 

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed
  3. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
RRID
AB_2561591 (BioLegend Cat. No. 141409)
AB_2561592 (BioLegend Cat. No. 141410)

Antigen Details

Structure
Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution
Many cell types, highly expressed on activated Tregs and platelets
Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor
TGF-β receptors
Cell Type
Tregs, Platelets, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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