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PerCP/Cyanine5.5 anti-mouse CD8b.2 Antibody

Pricing & Availability
Clone
53-5.8 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3.2, Ly-3.2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
53-58_PerCPCyanine55_CD8b2_Antibody_032519
C57BL/6 mouse splenocytes stained with CD3e FITC and CD8b.2 (clone 53-5.8) PerCP/Cyanine5.5 (left) or Rat IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • 53-58_PerCPCyanine55_CD8b2_Antibody_032519
    C57BL/6 mouse splenocytes stained with CD3e FITC and CD8b.2 (clone 53-5.8) PerCP/Cyanine5.5 (left) or Rat IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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140417 25 µg 100€
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140418 100 µg 278€
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
  2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
  3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
  4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
  5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations
  1. Yang N, et al. 2022. NPJ Vaccines. 7:120. PubMed
  2. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  3. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
RRID
AB_2800650 (BioLegend Cat. No. 140417)
AB_2800651 (BioLegend Cat. No. 140418)

Antigen Details

Structure
Ig superfamily, CD8β chain, 32 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8b.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 03.25.2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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