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PerCP/Cyanine5.5 anti-human Perforin Antibody

Pricing & Availability
Clone
B-D48 (See other available formats)
Regulatory Status
RUO
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
B-D48_PerCPCyanine55_Perforin_Antibody_120418
Human peripheral blood lymphocytes were surface stained with CD8 APC, then fixed, permeabilized, and intracellularly stained with Perforin (clone B-D48) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • B-D48_PerCPCyanine55_Perforin_Antibody_120418
    Human peripheral blood lymphocytes were surface stained with CD8 APC, then fixed, permeabilized, and intracellularly stained with Perforin (clone B-D48) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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353313 25 tests 141€
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353314 100 tests 306€
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Product Citations
  1. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  2. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  3. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  4. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_2571970 (BioLegend Cat. No. 353313)
AB_2571971 (BioLegend Cat. No. 353314)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis.
Cell Type
NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12.04.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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