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PerCP/Cyanine5.5 anti-human CD79a (Igα) Antibody

Pricing & Availability
Clone
HM47 (See other available formats)
Regulatory Status
RUO
Workshop
V cB017
Other Names
Mb-1, Iga, CD79
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HM47_PerCPCyanine55_CD79a_Antibody_1_060719
Human peripheral blood lymphocytes surface stained with CD19 APC, then fixed, permeabilized, and intracellularly stained with CD79a (Iga) (clone HM47) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • HM47_PerCPCyanine55_CD79a_Antibody_1_060719
    Human peripheral blood lymphocytes surface stained with CD19 APC, then fixed, permeabilized, and intracellularly stained with CD79a (Iga) (clone HM47) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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333508 100 tests 306€
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Description

CD79a is a 47 kD type I integral membrane protein, also known as mb-1 or Iga. It is a member of the Ig superfamily and disulphide-associated with CD79b (B29). The interaction of CD79a/CD79b heterodimer with B cell suface Ig forms B cell antigen receptor complex. CD79a is expressed in B cells from early pre-B to plasma cell stage. It has been shown that CD79a is also weakly expressed in some precursors of T- and myeloid cells. CD79 mediates the transport of IgM to B cell surface and transduces signals initiated by BCR aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

RRID
AB_2075752 (BioLegend Cat. No. 333508)

Antigen Details

Structure
47 kD, type I integral protein, Ig superfamily, CD79a/CD79b heterodimer interacts with B cell membrane Ig.
Distribution

B cells (from early pre-B to plasma cell stage), some T and myeloid cell precursors

Function
In association with CD79b and B cell membrane Ig to form B cell antigen receptor complex
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Astsaturov IA, et al. 1996. Leukemia 10:769
3. Mson DY, et al. 1995 Blood 86:1453
4. Hashimoto M, et al. 2002. J. Pathol. 197:341

Gene ID
973 View all products for this Gene ID
UniProt
View information about CD79a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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