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PerCP/Cyanine5.5 anti-human CD24 Antibody

Pricing & Availability
Clone
ML5 (See other available formats)
Regulatory Status
RUO
Workshop
V CD24.5
Other Names
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
ML5_PerCPCyanine55_CD24_Antibody_103118
Human peripheral lymphocytes were stained with CD24 (clone ML5) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, ? isotype control PerCP/Cyanine5.5 (open histogram).
  • ML5_PerCPCyanine55_CD24_Antibody_103118
    Human peripheral lymphocytes were stained with CD24 (clone ML5) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, ? isotype control PerCP/Cyanine5.5 (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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311115 25 tests 141€
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311116 100 tests 306€
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Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.

Additional Product Notes
View more applications data for this product in our Scientific Poster Library.

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
  4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
  1. Zheng H, et al. 2018. Hepatology. 68:127. PubMed
  2. Liu MY, et al. 2019. Oncol Lett. 18:3664. PubMed
  3. Cho A, et al. 2019. Cell Rep. 28:909. PubMed
  4. Sen U, et al. 2021. J Cell Biochem. 122:349. PubMed
  5. Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
RRID
AB_10962689 (BioLegend Cat. No. 311115)
AB_10960741 (BioLegend Cat. No. 311116)

Antigen Details

Structure
GPI-linked glycoprotein, 35-45 kD
Distribution

B cells, granulocytes, epithelial cells

Function
B cell proliferation and differentiation
Ligand/Receptor
CD62P (P-Selectin)
Cell Type
B cells, Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.

Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10.31.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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