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PerCP/Cyanine5.5 anti-human CD158b/j (KIR2DL2/L3/S2) Antibody

Pricing & Availability
Clone
DX27 (See other available formats)
Regulatory Status
RUO
Other Names
CD158b1 (KIR2DL2, p58.2), CD158b2 (KIR2DL3, p58.3), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
DX27_PerCPCyanine55_CD158b_Antibody_030519_updated.png
Human peripheral blood lymphocytes were stained with CD56 APC and CD158b (KIR2DL2/L3, NKAT2)(clone DX27) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right)
  • DX27_PerCPCyanine55_CD158b_Antibody_030519_updated.png
    Human peripheral blood lymphocytes were stained with CD56 APC and CD158b (KIR2DL2/L3, NKAT2)(clone DX27) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right)
Compare all formats See PerCP/Cyanine5.5 spectral data
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312613 25 tests 141€
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312614 100 tests 306€
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Description

CD158b is expressed on natural killer cells and a subset of T cells. It is a member of the immunoglobulin superfamily containing two immunoglobulin C2-type domains. Both variants and alternative isoforms of CD158b have been reported. The interaction of CD158b with specific HLA-C antigens on a target cell (HLA-Cw1, HLA-Cw3, HLA-Cw7 alleles, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK cell and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The DX27 monoclonal antibody reacts with a common epitope of KIR2DL2 (CD158b1, p58.2), KIR2DL3 (CD158b2, p58.3), and KIR2DS2 (CD158j, p50.2). Additional reported applications (for the relevant formats) include: restoring the NK cell cytotoxicity1,5.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  2. Lucas M, et al. 2003. J. Virol. 77:2251.
  3. Goodier M, et al. 2000. J. Immunol. 165:139.
  4. Yawata M, et al. 2002. Immunogenetics 54:543.
  5. Valiante NM, et al. 1997. Immunity 7:739.
Product Citations
  1. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
RRID
AB_2564334 (BioLegend Cat. No. 312613)
AB_2564335 (BioLegend Cat. No. 312614)

Antigen Details

Structure
Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, type I membrane protein
Distribution

NK cells and a subset of T cells

Function
Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-C antigens on target cell
Ligand/Receptor
HLA-C antigens such as HLA-Cw1, HLA-Cw3, HLA-Cw7
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. Uhrburg M, et al. 1997. Immunity 7:753.
3. Wagtmann N, et al. 1995. Immunity 3:801.
4. Dohring C, et al. 1996. Immunogenetics 44:227.
5. Maenaka K, et al. 1999. Structure 7:391.

Gene ID
3803 View all products for this Gene ID
UniProt
View information about CD158b/j on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 11.21.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD158b/j (KIR2DL2/L3/S2)

  • FITC anti-human CD158b/j (KIR2DL2/L3/S2)

  • PE anti-human CD158b/j (KIR2DL2/L3/S2)

  • APC anti-human CD158b/j (KIR2DL2/L3/S2)

  • PE/Cyanine7 anti-human CD158b/j (KIR2DL2/L3/S2)

  • PerCP/Cyanine5.5 anti-human CD158b/j (KIR2DL2/L3/S2)

  • TotalSeq™-A0592 anti-human CD158b/j (KIR2DL2/L3/S2)

  • TotalSeq™-B0592 anti-human CD158b/j (KIR2DL2/L3/S2)

  • APC/Fire™ 750 anti-human CD158b/j (KIR2DL2/L3/S2)

  • TotalSeq™-C0592 anti-human CD158b/j (KIR2DL2/L3/S2)

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