- W6/32 (See other available formats)
- Regulatory Status
- Other Names
- Major Histocompatibility Class I, MHC class I
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.Product Details
- Human, African Green, Baboon, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.
Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Darrow TL, et al. 1989. J. Immunol. 142:3329.
- Stern P, et al. 1987. J. Immunol. 138:1088.
- Tran TM, et al. 2001. Immunogenetics 53:440.
- Barbatis C, et al. 1981. Gut 22:985.
- Ayyoub M, et al. 2004. Cancer Immunity 4:7.
- DeFelice M, et al. 1990. Cell. Immunol. 126:420.
- Fayen J, et al. 1998. Int. Immunol. 10:1347.
- Turco MC, et al. 1988. J. Immunol. 141:2275.
- Geppert TD, et al. 1989. J. Immunol. 142:3763.
- Wooden SL, et al. 2005. J. Immunol. 175:1383.
- Nagano M, et al. 2007. Blood 110:151.
- McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
- Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
- Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
- Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
- Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
- Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
- Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
- Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
- Product Citations
AB_1501265 (BioLegend Cat. No. 311421)
AB_1501263 (BioLegend Cat. No. 311422)
- Ig superfamily
All nucleated cells
- Antigen presentation
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 3105 View all products for this Gene ID
- View information about HLA-A,B,C on UniProt.org
- How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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