Loading...

PerCP anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
HIT3a_PerCP_CD3_Antibody_052316
Human, lysed whole blood was stained with CD3 (clone HIT3a) PerCP (filled histogram) or Mouse IgG2a, κ PerCP isotype control (open histogram).
  • HIT3a_PerCP_CD3_Antibody_052316
    Human, lysed whole blood was stained with CD3 (clone HIT3a) PerCP (filled histogram) or Mouse IgG2a, κ PerCP isotype control (open histogram).
Compare all formats See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
300325 25 tests 109€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300326 100 tests 221€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Zheng G, et al. 2021. Signal Transduct Target Ther. 6:236. PubMed
  2. Zhang H, et al. 2021. Front Immunol. 12:644520. PubMed
  3. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  4. Lu Y, et al. 2019. Anticancer Res. 39:5911. PubMed
  5. DeKosky B, et al. 2016. Proc Natl Acad Sci U S A. 113: 2636 - 2645. PubMed
  6. Sekhri P, et al. 2022. Cancers (Basel). 14:. PubMed
  7. Arora JK, et al. 2022. iScience. 25:104034. PubMed
  8. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  9. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  10. Chen Z, et al. 2021. Dis Markers. 2021:5838582. PubMed
  11. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  12. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  13. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  14. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_2616609 (BioLegend Cat. No. 300325)
AB_2616610 (BioLegend Cat. No. 300326)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Customers Also Purchased

APC anti-human IFN-γ
Clone: B27

PE anti-human CD8
Clone: SK1

FITC anti-human CD25
Clone: BC96

FITC anti-human CD19
Clone: HIB19

Go To Top Version: 1    Revision Date: 05.24.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3

  • Biotin anti-human CD3

  • FITC anti-human CD3

  • PE anti-human CD3

  • PE/Cyanine5 anti-human CD3

  • Purified anti-human CD3

  • APC/Cyanine7 anti-human CD3

  • PE/Cyanine7 anti-human CD3

  • Alexa Fluor® 488 anti-human CD3

  • Alexa Fluor® 647 anti-human CD3

  • Alexa Fluor® 700 anti-human CD3

  • PerCP anti-human CD3

  • PerCP/Cyanine5.5 anti-human CD3

  • Pacific Blue™ anti-human CD3

  • Ultra-LEAF™ Purified anti-human CD3

  • PE/Dazzle™ 594 anti-human CD3

  • Spark UV™ 387 anti-human CD3

  • Spark Red™ 718 anti-human CD3

  • TotalSeq™-C1290 anti-human CD3

  • TotalSeq™-B1290 anti-human CD3

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account