PE/Dazzle™ 594 anti-human B7-H4 Antibody

Pricing & Availability
MIH43 (See other available formats)
Regulatory Status
Other Names
VTCN1, B7h.5
Mouse IgG1, κ
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Product Citations
Human B7-H4 transfected P815 cells were stained with B7-H4 (clone MIH43) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • MIH43_PEDZL594_B7-H4_Antibody_FC_092514
    Human B7-H4 transfected P815 cells were stained with B7-H4 (clone MIH43) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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358112 100 tests 306€
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B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) includes immunofluorescence2.

Application References

(PubMed link indicates BioLegend citation)
  1. Quandt D, et al. 2011. Clin. Cancer Res. 17:3100.
  2. Smith JB, et al. 2014. Gynecol. Oncol. 134:181. (FC, IF)
AB_2564337 (BioLegend Cat. No. 358112)

Antigen Details

Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain

Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian

Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Antigen References

1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
2. Chen C, et al. 2012. J. Immunother. 35:354.
3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748.

Gene ID
79679 View all products for this Gene ID
View information about B7-H4 on

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Go To Top Version: 2    Revision Date: 05.05.2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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