PE/Cyanine7 Streptavidin

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Other Names
SAv-PE/Cyanine7
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405206 100 µg 140€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine7 by 488 nm laser light induces a light emission maximum of 775 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-PE/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine7 conjugation.  For immunofluorescent staining, the recommended use of this reagent is =0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin - Phycoerythrin/Cyanine7 (PE/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine7 is 360 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
  1. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  2. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
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  17. Finke S 2010. J Immunol. 184:3562. PubMed
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  19. Ahuja A, et al. 2011. J Immunol. 187:3888. PubMed
  20. Weber S, et al. 2011. J Immunol. 186:432. PubMed
  21. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  22. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  23. Rigolio R, et al. 2008. J Neuroimmunol. 199:67. PubMed
  24. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  25. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  26. Matsumura M 2014. Sci Rep. 4:4852. PubMed
  27. Nakamura M, et al. 2014. Mult Scler. 20:1371. PubMed
  28. Shaw O, et al. 2014. Rheumatology. 53:1901. PubMed
  29. Rando A 2014. EMBO J. 33:2782. PubMed
  30. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
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  40. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
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  42. Zheng H, et al. 2017. Sci Rep. 7:37. PubMed

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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