PE/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279
Isotype
Rat IgG2b, κ
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Product Citations
publications
RMP1-30_PECy7_021808
Con A-stimulated (day-3) Balb/c mouse splenocytes stained with RMP1-30 PE/Cyanine7
  • RMP1-30_PECy7_021808
    Con A-stimulated (day-3) Balb/c mouse splenocytes stained with RMP1-30 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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109109 25 µg 80,00€
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109110 100 µg 212,00€
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD279 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Product Citations
  1. Nawaf M, et al. 2017. J Immunol. 10.4049/jimmunol.1700088. PubMed
  2. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  3. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  4. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  5. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  6. Shi J, et al. 2018. Immunity. 49:264. PubMed
  7. Verghese DA, et al. 2018. JCI Insight. 3:. PubMed
  8. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  9. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  10. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  11. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  12. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  13. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  14. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
  15. Myers DR et al. 2019. Cell reports. 27(6):1858-1874 . PubMed
  16. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  17. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  18. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  19. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  20. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
  21. Singer M et al. 2016. Cell. 166(6):1500-1511 . PubMed
  22. Filskov J, et al. 2019. Sci Rep. 9:14085. PubMed
  23. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  24. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  25. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  26. Sullivan JA, et al. 2020. Cell Rep. 30:1039. PubMed
  27. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  28. Jin C, et al. 2019. Cell. 176:998. PubMed
  29. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  30. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  31. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  32. Mohrs I 2009. J Exp Med. 206:1001. PubMed
  33. Lindenstrøm T, et al. 2013. J Immunol. 190:6311. PubMed
  34. Jeon S, et al. 2013. J Immunol. 190:6277. PubMed
  35. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  36. Woodworth J, et al. 2014. J Immunol. 192:3247. PubMed
  37. Christensen D, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.28. PubMed
  38. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  39. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  40. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  41. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  42. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  43. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  44. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  45. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  46. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  47. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  48. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  49. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  50. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  51. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  52. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  53. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
RRID
AB_572016 (BioLegend Cat. No. 109109)
AB_572017 (BioLegend Cat. No. 109110)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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