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PE/Cyanine5 anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
MEM-188 (See other available formats)
Regulatory Status
RUO
Workshop
VI NK26
Other Names
NCAM, Leu-19, NKH1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MEM-188_PECy5_070207
Human peripheral blood lymphocytes stained with MEM-188 PE/Cyanine5
  • MEM-188_PECy5_070207
    Human peripheral blood lymphocytes stained with MEM-188 PE/Cyanine5
Compare all formats See PE/Cyanine5 spectral data
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304607 25 tests 113€
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304608 100 tests 244€
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Description

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG-1 human acute myelogenous leukemia cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations
  1. Buffone A, et al. 2018. J Cell Sci. 131:. PubMed
  2. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  3. Stremming J, et al. 2022. Front Physiol. 13:954948. PubMed
  4. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  5. Chang EI, et al. 2021. Front Endocrinol (Lausanne). 12:785242. PubMed
  6. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  7. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
RRID
AB_314449 (BioLegend Cat. No. 304607)
AB_314450 (BioLegend Cat. No. 304608)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.16.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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