PE anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C6B05 (See other available formats)
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7C6B05_PE_SPI1_Antibody_1_051616
Human peripheral blood lymphocytes were surface stained with CD19 Alexa Fluor® 647 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-human SPI1 (clone 7C6B05) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 7C6B05_PE_SPI1_Antibody_1_051616
    Human peripheral blood lymphocytes were surface stained with CD19 Alexa Fluor® 647 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-human SPI1 (clone 7C6B05) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 7C6B05_PE_SPI1_Antibody_2_051616
See PE spectral data
Cat # Size Price Quantity Avail. Save
658009 25 tests 104€
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658010 100 tests 244€
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Description

SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death.  Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SPI1 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2616862 (BioLegend Cat. No. 658009)
AB_2616863 (BioLegend Cat. No. 658010)

Antigen Details

Structure
270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution
Nucleus.
Function
SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.

Gene ID
6688 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.18.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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