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PE anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5/114.15.2
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
  • M5/114.15.2
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
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107607 50 µg 74€
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107608 200 µg 264€
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  2. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  3. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  4. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  5. Shi L, et al. 2019. Theranostics. 9:4115. PubMed
  6. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  7. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  8. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  9. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  10. Zhu J, et al. 2016. Sci Rep. 6:27136. PubMed
  11. Yasmin N, et al. 2013. J Exp Med. 210:2597. PubMed
  12. Blumenthal D, et al. 2020. eLife. 9:e55995.. PubMed
  13. Cai W, et al. 2020. J Immunol Res. 2019:2835256. PubMed
  14. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  15. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  16. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  17. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  18. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  19. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  20. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  21. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  22. Dhandapani R, et al. 2018. Nat Commun. 9:1640. PubMed
  23. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  24. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  25. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  26. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  27. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  28. Tu J, et al. 2022. Theranostics. 12:747. PubMed
  29. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  30. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  31. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  32. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  33. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  34. Novkovic M, et al. 2016. PLoS Biol. 14: 1002515. PubMed
  35. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  36. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  37. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  38. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  39. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  40. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  41. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  42. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  43. Liu J, et al. 2020. Nat Commun. 11:6396. PubMed
  44. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  45. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  46. Morales-Kastresana A, et al. 2020. Curr Protoc Cytom. 95:e81. PubMed
  47. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  48. Pazmandi K, et al. 2014. Free Radic Biol Med. 77:281. PubMed
  49. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
  50. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  51. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  52. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  53. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  54. Huang L, et al. 2021. Frontiers in Cellular and Infection Microbiology. 10:606340. PubMed
  55. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  56. Zhao H, et al. 2022. Int J Biol Sci. 18:154. PubMed
  57. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  58. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  59. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  60. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  61. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  62. Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed
  63. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  64. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  65. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
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RRID
AB_313322 (BioLegend Cat. No. 107607)
AB_313323 (BioLegend Cat. No. 107608)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10.30.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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