- QA20A67 (See other available formats)
- Regulatory Status
- Other Names
- Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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FOXP3, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX, is a 47 kD transcription factor. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone QA20A67 can completely block the binding of clone MF-14 on target cells.
AB_2936574 (BioLegend Cat. No. 118904)
- Forkhead/winged-helix transcription factor
T regulatory cells
- Transcription Factor
- Cell Type
- Lymphocytes, T cells, Tregs
- Biology Area
- Immunology, Transcription Factors
- Antigen References
- Ono M, et al. 2007. Nature. 446:685-9.
- Hori S, et al. 2003. Science. 299:1057-61.
- Fontenot JD, et al. 2003. Nat Immunol. 4:330-6.
- Gene ID
- 20371 View all products for this Gene ID
- View information about FOXP3 on UniProt.org
Related Pages & Pathways
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
- Can FOXP3 be costained with cytokines?
The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.
Other FormatsView All FOXP3 Reagents Request Custom Conjugation
|PE anti-mouse FOXP3 Recombinant Antibody||QA20A67||ICFC|
|Purified anti-mouse FOXP3 Recombinant Antibody||QA20A67||ICFC|
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