PE anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_PE_032309.jpg
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
  • 16-10A1_PE_032309.jpg
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
See PE spectral data
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104707 50 µg 72,00€
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104708 200 µg 200,00€
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Cross-Reactivity: Dog (Canine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Simmons D, et al. 2012. J Immunol. 188:3116. PubMed
  2. Marshall N, et al. 2012. Cancer Res. 72:581. PubMed
  3. Jeisy-Scott V, et al. 2012. J Virol. 86:10988. PubMed
  4. Wong E, et al. 2012. J Immunol. 189:5667. PubMed
  5. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  6. White C, et al. 2015. J Immunol. 194:697. PubMed
  7. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  8. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  9. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  10. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  11. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  12. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  13. Kadir NA, et al. 2020. Pathogens. 9:00. PubMed
  14. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  15. Feng S, et al. 2021. Exp Ther Med. 21:20. PubMed
  16. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  17. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  18. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  19. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  20. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  21. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  22. Zhang F, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.019. PubMed
  23. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  24. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  25. Xie Z, et al. 2018. J Am Heart Assoc. 7:e007442. PubMed
  26. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  27. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  28. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  29. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  30. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  31. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  32. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  33. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  34. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  35. Matundan H, et al. 2019. J Virol. 93. PubMed
  36. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  37. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  38. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  39. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  40. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  41. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  42. Yang C, et al. 2019. Nanomedicine (Lond). 14:2423. PubMed
  43. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  44. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  45. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  46. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  47. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  48. Rozanski C, et al. 2011. J Exp Med. 208:1435. PubMed
RRID
AB_313128 (BioLegend Cat. No. 104707)
AB_313129 (BioLegend Cat. No. 104708)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 2    Revision Date: 02.18.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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