- IK22/5 (See other available formats)
- Other Names
- Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
- Rat IgG2a, κ
- Ave. Rating
- 2 reviews
- Product Citations
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CD254 is a 19 kD TNF superfamily member also known as TRANCE (TNF-related activation induced cytokine), RANK ligand, RANKL, TNFSF11, OPGL, and ODF. TRANCE is expressed on activated T cells and osteoclasts and has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation.Product Details
- Antibody Type
- Host Species
- NSO-derived recombinant mouse TRANCE
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 510008).
(PubMed link indicates BioLegend citation)
- Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
- Personal communication (Block IP WB)
- Gao Y, et al. 2007. J. Clin. Invest. 117:122.
- Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
- Fionda C, et al. 2007. J. Immunol. 178:4039.
- Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
- Takeshita S, et al. 2014. J Biol Chem. 289:16699. PubMed
- Product Citations
AB_315553 (BioLegend Cat. No. 510005)
AB_2303587 (BioLegend Cat. No. 510006)
- TNF superfamily; 19 kD (Mammalian)
- Activated T cells, osteoclasts
- Regulates T and dendritic cell interactions, and osteoclast differentiation
- RANK, OPG
- Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
- Cell Sources
- Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
- Cell Targets
- Dendritic cells, osteoclasts
- ODFR, ODAR, TRANCE-R
- Cell Type
- T cells, Osteoclasts
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.
- Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
- Gene ID
- 21943 View all products for this Gene ID
- View information about CD254 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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