- 55R-286 (See other available formats)
- Other Names
- TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
- Armenian Hamster IgG
- Ave. Rating
- 2 reviews
- Product Citations
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CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- E. coli-expressed extracellular domain of the mouse TNFRI protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.
(PubMed link indicates BioLegend citation)
- Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP)
- Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
- Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)
- Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)
- Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.
- Wang K, et al. 2012. PLoS One. 7:e52924. PubMed
- Pereira IR, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed
- Product Citations
AB_313532 (BioLegend Cat. No. 113003)
AB_313533 (BioLegend Cat. No. 113004)
- TNFR superfamily, 55 kD
Variety of cell types at low levels
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- TNF-α, LT-α (TNF-β)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.
- Gene ID
- 21937 View all products for this Gene ID
- View information about CD120a on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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