PE anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
W19330E (See other available formats)
Regulatory Status
RUO
Other Names
CSF-1R, M-CSR, c-fms
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19330E_PE_CD115_Antibody_022422
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with PE anti-mouse CD115 (clone W19330E) (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • W19330E_PE_CD115_Antibody_022422
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with PE anti-mouse CD115 (clone W19330E) (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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165203 25 µg 76€
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD115 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W19330E does not affect binding of clone W19330C on target cells.

Clone W19330E can block clone AFS98 binding on target cells.   

Clone W19330E can block ligand binding to CSFR-1.

RRID
AB_2910352 (BioLegend Cat. No. 165203)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts 

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Guilbert LJ and Stanley ER. 1980. J Cell Bio. 85:153-9.
  2. Yeung Y-G, et al. 1978. Proc Natl Acad Sci. 84:1268-71.
  3. Sherr CJ, et al. 1985. Cell. 41:665-76.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.24.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD115 (CSF-1R)

  • PE anti-mouse CD115 (CSF-1R)

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