PE anti-marmoset CD45 Antibody

Pricing & Availability
Clone
6C9 (See other available formats)
Other Names
LCA, T200
Isotype
Mouse IgG1
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Product Citations
publications
6C9_PE_Marmoset_CD45_Antibody_FC_052413
Marmoset peripheral blood lymphocytes were stained with CD45 (clone 6C9) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 6C9_PE_Marmoset_CD45_Antibody_FC_052413
    Marmoset peripheral blood lymphocytes were stained with CD45 (clone 6C9) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
250204 100 µg 172€
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Reactivity
Marmoset
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Marmoset mononuclear cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen spleen sections1 and Western blotting1. The 6C9 antibody has been reported to not cross-react with humans, cynomolgus monkeys, or squirrel monkeys.

Application References

(PubMed link indicates BioLegend citation)
  1. Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, WB)
Product Citations
  1. Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed
Publication Library
RRID
AB_2562120 (BioLegend Cat. No. 250204)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein
Distribution

Hematopoietic cells, except circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I and Thomas M. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
100385061 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.24.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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