- 546-16 (See other available formats)
- Regulatory Status
- Other Names
- MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. HIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α. Following nuclear translocation, HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE); only the HIF1 heterodimer can bind DNA. HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in angiogenesis and energy metabolism.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant HIF1α
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
AB_2562422 (BioLegend Cat. No. 359703)
AB_2562423 (BioLegend Cat. No. 359704)
- 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains
- Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation
- Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations
- Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304.
2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513.
3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230.
4. Galbraith MD, et al. 2013. Cell. 153:1327.
- Gene ID
- 3091 View all products for this Gene ID
- View information about HIF1a on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
|Alexa Fluor® 647 anti-human HIF1α||546-16||ICFC|
|PE anti-human HIF1α||546-16||ICFC|
|Alexa Fluor® 488 anti-human HIF1α||546-16||ICFC|
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