PE anti-human FCGR2A/CD32A Antibody

Pricing & Availability
Clone
S20004A (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIIa
Isotype
Mouse IgG1, κ
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Product Citations
publications
S20004A_PE_FCGR2A_CD32A_Antibody_111721
Human peripheral blood monocytes stained with anti-human FCGR2A/CD32A (clone S20004A) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • S20004A_PE_FCGR2A_CD32A_Antibody_111721
    Human peripheral blood monocytes stained with anti-human FCGR2A/CD32A (clone S20004A) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
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365503 25 tests 76,00€
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Description

CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes.  FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells; CD32a is also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32a is involved in phagocytosis and clearing of immune complexes.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FCGR2A-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

 This clone does not recognizes CD32B-transfected cell

Product Citations

Antigen Details

Structure
Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
Distribution

Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells

Function
Phagocytosis, cytotoxicity, platelet aggregation
Ligand/Receptor
Aggregated IgG, immune complexes
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
Biology Area
Adaptive Immunity, Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hubbard J. et al. 2020. J Exp Med. 217:e20200359
  2. Newling M, et al. 2020. Clin Exp Immunol. 199:39-49
  3. Maria H, et al. 2018. Front Immunol. 9:2814
  4. Descours B, et al. 2017. Nature. 543:564-567
Gene ID
NA
UniProt
View information about FCGR2A/CD32A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.17.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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