PE anti-human CD85j (ILT2) Antibody

Pricing & Availability
Clone
GHI/75 (See other available formats)
Workshop
V B032
Other Names
ILT2, LIR-1, LILRB1, CD85
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
GHI_75_PE_090808.jpg
Human peripheral blood lymphocytes stained with GHI/75 PE
  • GHI_75_PE_090808.jpg
    Human peripheral blood lymphocytes stained with GHI/75 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
333707 25 tests 96€
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333708 100 tests 208€
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Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85j is the 110kD member, known as ILT2, LIR1, or LILRB1, and MIR7. ILT2 structurally has four Ig domains and contains ITIMs in its cytoplasmic tail that provide inhibitory signals by recruiting SHP-1. ILT2 is found on the surface of B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells. The ligands of ILT2 include a broad range of HLA-A, -B molecules, some HLA-C and HLA-G molecules, and the human cytomegalovirus UL18 protein.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application: Block HLA-G induced TGF-β1 production.

Application References

(PubMed link indicates BioLegend citation)
  1. Pulford K, et al. 1991. Clin. Exp. Immunol. 85:429
  2. Mclntire RH, et al. 2004. J. Leukoc. Biol. 76:1220
Product Citations
  1. Morandi B, et al. 2013. J Immunol. 191:4858. PubMed
  2. Dauby N, et al. 2014. J Infect Dis. 210:1275. PubMed
  3. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
Publication Library
RRID
AB_1089086 (BioLegend Cat. No. 333707)
AB_2136385 (BioLegend Cat. No. 333708)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 110 kD
Distribution

B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells.

Function
Inhibitory receptor for MHC class I
Ligand/Receptor
HLA-A, -B, some HLA-C and G molecules, human cytomegalovirus UL18 protein
Cell Type
B cells, Dendritic cells, Monocytes, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006

Gene ID
10859 View all products for this Gene ID
UniProt
View information about CD85 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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