PE anti-human CD289 (TLR9) Antibody

Pricing & Availability
Clone
S16013D (See other available formats)
Other Names
CD289, TLR9, Toll-like receptor 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16013D_PE_CD289_antibody_1_011819
Human peripheral blood mononuclear cells were stained with CD304 Alexa Fluor® 647 and CD19 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) PE (left) or Rat IgG2a, κ PE isotype control (right). Dot plots exclude monocytes.
  • S16013D_PE_CD289_antibody_1_011819
    Human peripheral blood mononuclear cells were stained with CD304 Alexa Fluor® 647 and CD19 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) PE (left) or Rat IgG2a, κ PE isotype control (right). Dot plots exclude monocytes.
  • S16013D_PE_CD289_antibody_2_011819
    Human peripheral blood mononuclear cells were stained with CD304 Alexa Fluor® 647 and CD19 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) PE (left) or Rat IgG2a, κ PE isotype control (right). Dot plots exclude monocytes.
  • S16013D_PE_CD289_antibody_3_020519
    Human peripheral blood was stained with CD304 Alexa Fluor® 647, CD19 Pacific Blue™, and CD289 (clone S16013D) PE (left) or Rat IgG2a, κ PE isotype control (right). Dot plots exclude monocytes and granulocytes.
See PE spectral data
Cat # Size Price Quantity Avail. Save
394803 25 tests 116€
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394804 100 tests 236€
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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD289 (TLR9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC, FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).

Antigen Details

Distribution

TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).

Ligand/Receptor
TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Hornung V, et al. 2002. J. Immunol. 168:4531.
  2. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202.
  3. Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID
54106 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01.14.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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