PE anti-human CD28 Antibody

Pricing & Availability
S20013B (See other available formats)
Regulatory Status
Other Names
T44, Tp44
Mouse IgG1, κ
Ave. Rating
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Product Citations
Human peripheral blood lymphocytes were stained with anti-human CD28 (clone 20013B) PE (left) or mouse IgG1, κ PE isotype control (right). Cells were costained with anti-human CD3 FITC.
  • 20013B_PE_CD28_Antibody_12072022
    Human peripheral blood lymphocytes were stained with anti-human CD28 (clone 20013B) PE (left) or mouse IgG1, κ PE isotype control (right). Cells were costained with anti-human CD3 FITC.
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Cat # Size Price Quantity Check Availability Save
377809 25 tests 85€
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377810 100 tests 188€
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CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human CD28 transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S20013B antibody can completely block the binding of clone CD28.2 on target.

Clone S20013B can partially block Clone S20013F on target.

Clone S20013B can increase anti-CD3 induced human peripheral T-cell proliferation.

Clone S20013B is a superagonistic antibody raised towards human CD28, and can induce activation, proliferation of human peripheral T cells without anti-CD3 stimulation.

AB_2928024 (BioLegend Cat. No. 377809)
AB_2928024 (BioLegend Cat. No. 377810)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

T cell costimulation
CD80, CD86
Cell Type
Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
Gene ID
940 View all products for this Gene ID
View information about CD28 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.07.2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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