PE anti-human CD158e1 (KIR3DL1, NKB1) Antibody

Pricing & Availability
Clone
DX9 (See other available formats)
Other Names
NKAT3, NKB1, KIR-NKB1, p70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DX9_PE_071504
Human peripheral blood lymphocytes stained with DX9 PE and CD16 FITC
  • DX9_PE_071504
    Human peripheral blood lymphocytes stained with DX9 PE and CD16 FITC
See PE spectral data
Cat # Size Price Quantity Avail. Save
312707 25 tests 80€
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312708 100 tests 176€
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Description

CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell clone VL186-1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 312710).

Application References

(PubMed link indicates BioLegend citation)
  1. Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
  2. Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
  3. Gardiner CM, et al. 2001. J. Immunol. 166:2992.
  4. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  5. Goodier M, et al. 2000. J. Immunol. 165:139.
  6. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
  7. Yawata M, et al. 2002. Immunogenetics 54:543.
  8. Valiante NM, et al. 1997. Immunity 7:739.
  9. Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
  10. Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
  11. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  12. Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
Product Citations
  1. Thomas R, et al. 2008. J Immunol. 180:6743. PubMed
  2. O’Connor G, et al. 2011. J Immunol. 187:2162. PubMed
  3. Berg M, et al. 2008. Cytotherapy. 11:341. PubMed
  4. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  5. Purdy A, et al. 2014. J Immunol. 193:4675. PubMed
Publication Library
RRID
AB_314946 (BioLegend Cat. No. 312707)
AB_2249498 (BioLegend Cat. No. 312708)

Antigen Details

Structure
Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Distribution

Subset of NK and T cells

Function
Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
Ligand/Receptor
HLA-B antigens such as the HLA-Bw4 allele
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.

Gene ID
3811 View all products for this Gene ID
UniProt
View information about CD158e1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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