PE anti-human CD11b (activated) Antibody

Pricing & Availability
Clone
CBRM1/5 (See other available formats)
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
CBRM1/5
PMA activated human granulocytes stained with CBRM1/5 FITC
  • CBRM1/5
    PMA activated human granulocytes stained with CBRM1/5 FITC
See PE spectral data
Cat # Size Price Quantity Avail. Save
301405 25 tests 80€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
301406 100 tests 184€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the α chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9.

Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301408).

Application References

(PubMed link indicates BioLegend citation)
  1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block)
  2. Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed
  3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed
  5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed
  6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed
  7. Koleva RI, et al. 2012. Blood. 119:4878. PubMed.
  8. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed
  9. Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215.
  10. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed
  11. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations
  1. Koleva R, et al. 2012. Blood. 119:4878. PubMed
  2. Eleftheriou D, et al. 2012. Neurology. 79:2089. PubMed
  3. K K, et al. 2016. Haematologica. 151159. PubMed
  4. Wang T 2018. Immunity. 49:504. PubMed
Publication Library
RRID
AB_314169 (BioLegend Cat. No. 301405)
AB_314170 (BioLegend Cat. No. 301406)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690.
2. Petty H, et al. 1996. Immunol. Today 17:209.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04.01.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account