Monensin Solution (1,000X)

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420701 1 mL 40€
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Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical data sheet

Product Details

Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
2.0 mM
Storage & Handling
Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.

ICFC - Quality tested

Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.

Application References

(PubMed link indicates BioLegend citation)
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Durkin ET,et al. 2008. Blood 10:1182.PubMed
  4. Durkin ET, et al. 2008. Blood 112:5245. PubMed
  5. Liu XS, et al. 2009. J. Immunol. 183:51. PubMed
  6. Mattarollo SR, et al. 2010. J. Immunol. 184:1242. PubMed
  7. Yang M, et al. 2010. J. Immuonl. 184:3321. PubMed
  8. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. PubMed
  9. Wood MW, et al. 2011. Infect Immun. 79:708. PubMed
  10. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
  11. Fiorenza S, et al. 2012. J. Immunol. 189:5622. PubMed
  12. Asgari E, et al. 2013. Blood. 122:3473. PubMed
  13. Li D, et al. 2013. Clin Immunol. 149:411. PubMed
  14. Bunse CE, et al. 2013. PLoS One. 8:77925. PubMed
Product Citations
  1. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  2. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  3. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  4. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  5. Bailey S, et al. 2017. Nat Commun.. 10.1038/s41467-017-01867-9. PubMed
  6. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  7. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  8. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  9. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  10. Mandour MF, et al. 2020. Infect Agent Cancer. 15:30. PubMed
  11. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  12. Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
  13. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  14. Durkin E, et al. 2008. Blood. 112:5245. PubMed
  15. Liu X, et al. 2009. J Immunol. 183:51:00. PubMed
  16. Mattarollo S, et al. 2010. J Immunol. 184:5663. PubMed
  17. Yang M, et al. 2010. J Immunol. 184:3321. PubMed
  18. Mattarollo S, et al. 2010. J Immunol. 184:1242. PubMed
  19. Wood M, et al. 2011. Infect Immun. 79:708. PubMed
  20. Munk R, et al. 2011. PLoS One. 6:e18553. PubMed
  21. Fiorenza S, et al. 2012. J Immunol. 189:5622. PubMed
  22. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
  23. Asgari E, et al. 2013. Blood. 122:3473. PubMed
  24. Li D, et al. 2013. Clin Immunol. 149:411. PubMed
  25. Bunse C, et al. 2013. PLoS One. 8:77925. PubMed
  26. Ren Y, et al. 2015. Biochim Biophys Acta. Available online 14 August 2015. PubMed
  27. Dimeloe S, et al. 2016. J Immunol. 196: 106 - 114. PubMed
  28. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  29. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  30. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
  31. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  32. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  33. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  34. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  35. Andrew Mansour, Laurentiu Pop, Ellen Vitetta 2016. PLoS One. 11:e0167618. PubMed
  36. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  37. Marvin S, et al. 2017. J Virol. 10.1128/JVI.01417-16. PubMed
  38. Theeb W, et al. 2017. Infect Immun . 10.1128/IAI.00475-16. PubMed
  39. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  40. Meyer C, Key P 2017. Imm Inflam Dis. 10.1002/iid3.156. PubMed
  41. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  42. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  43. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  44. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  45. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID

Related FAQs

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

Go To Top Version: 1    Revision Date: 11.30.2012

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