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A single cell suspension from human monocyte depleted peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD4+ cells using the MojoSort™ Human CD4 Nanobeads. Cells were stained with CD4 (clone OKT4) PE and CD3 (clone UCHT1) APC. -
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480013 | 10 tests | 120,00€ | ||||
| 480014 | 100 tests | 360,00€ | ||||
CD4+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD4 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
-
For Cat# 480013:
- 1 vial containing 100 µl CD4 nanobeads
For Cat# 480014:
- 1 vial containing 1 ml of CD4 Nanobeads
Product Details
- Reactivity
- Human
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Human CD4 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Human CD4 Nanobeads for 1x107 cells in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive isolation of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs). To maximize the purity of CD4+ T cells, we strongly recommend depletion of monocytes prior to isolation. Efficient isolation of monocytes can be achieved using Biotin anti-human CD14 antibody and Streptavidin Nanobeads.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 5X
To find out the dilution to use this product in separation columns please download the CoA. - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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