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Other Names
IL-6 Pre-coated ELISA Kit
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Product Citations
IL-6 061709
  • IL-6 061709
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431307 1 Pre-coated Plate 281€
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Mouse IL-6 (other names include B-cell Stimulatory Factor 2, Interferon Beta-2, Hybridoma Growth Factor, and Cytotoxic T-Cell Differentiation Factor) plays an essential role in the final differentiation of B-cells into imunoglobin-secreting cells, it induces myeloma and plasmacytoma growth, induces nerve cell differentiation, and in hepatocytes it induces acute phase reactant proteins. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Current research supports IL-6 as playing a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis.

BioLegend’s LEGEND MAX™ Mouse IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-6 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IL-6ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-6 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse IL-6 Pre-coated 96-well Strip Microplate
  • Mouse IL-6 Dectection Antibody
  • Mouse IL-6 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution E
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Application References

(PubMed link indicates BioLegend citation)
  1. Liang G, et al. 2009. Eur J Med Chem. 44:915. PubMed
Product Citations
  1. Lliberós C, et al. 2021. Front Cell Dev Biol. 8:628473. PubMed
  2. Shi Z, et al. 2022. Br J Pharmacol. 179:5209. PubMed
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  4. Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed
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  9. Sun Q, et al. 2023. Transl Neurosci. 14:20220273. PubMed
  10. Liang G, et al. 2009. Eur J Med Chem. 44:915. PubMed
  11. Afraei S, et al. 2018. Cent Eur J Immunol. 43:9. PubMed
  12. Du L, et al. 2022. Aging (Albany NY). 14:6626. PubMed
  13. Au DT, et al. 2018. Mediators Inflamm. 2018:7902841. PubMed
  14. Yin Y, et al. 2022. Front Pharmacol. 13:882924. PubMed
  15. Wu D, et al. 2022. Cell Death Dis. 13:731. PubMed
  16. Lliberós C, et al. 2021. Sci Rep. 0.651388889. PubMed
  17. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  18. Wu H, et al. 2019. Autophagy. :1. PubMed
  19. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  20. Kessler SP, et al. 2018. J Histochem Cytochem. 66:273. PubMed
  21. Xu X, et al. 2018. Cell. 175:1336. PubMed
  22. Assi M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.158. PubMed
  23. Chen H, et al. 2021. Invest Ophthalmol Vis Sci. 62:11. PubMed
  24. Tian M, et al. 2020. Cell Rep. 108297:33. PubMed
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  30. Wang X, et al. 2022. Front Cell Infect Microbiol. 12:839592. PubMed
2 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Gene ID
16193 View all products for this Gene ID
View information about IL-6 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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