LEGEND MAX™ Human Total MMP-2 ELISA Kit

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Other Names
Matrix Metallo proteinase 2, Gelatinase A, 72kD Type IV Collagenase, CLG4A
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MMP-2_Human_Total_ELISA_Kit_LegendMax_122216
  • MMP-2_Human_Total_ELISA_Kit_LegendMax_122216
Cat # Size Price Quantity Save
444607 1 Pre-coated Plate 340€
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Description

Matrix metalloproteinase 2 (MMP-2), also known as gelatinase A or 72 kD type IV collagenase, is a member of a broad family of MMPs, which are zinc-dependent enzymes that break down extracellular matrix proteins as part of their primary functions. MMP-2 is secreted in its 72 kD latent pro form from many cell types including neutrophils, monocytes, macrophages, endothelial cells, platelets, and tumor cells. It exists as a monomer and can form a homodimer that is known to regulate pro-MMP- 2 activation. When its pro-domain is cleaved by proteases such as MMP-14 and thrombin, it becomes the active 62 kD mature form. Activated MMP-2 degrades collagenase of the basement membrane and gelatin as well as several chemokines and cytokines. MMP-2 can also bind to other proteins, such as the tissue inhibitors of metalloproteinase (TIMPs). Specifically, TIMP-2 is able to bind to MMP-2 and MMP-14 to form a complex that regulates MMP-2 activation on the cell surface.

MMP-2 is closely related to MMP-9, although it only shares 46% amino acid sequence identity. Human MMP-2 shares significant {>95%) amino acid sequence identity with MMP-2 from other species, including non­human primates, equine, porcine, mouse, bovine, and rat.

MMP-2 plays important roles in vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP-2 is a potential biomarker for many diseases and conditions, as it is involved in tumor metastasis, inflammatory conditions, allergies, autoimmune diseases, and myocardial infarction.

The LEGEND MAX™ Human Total MMP-2 Sandwich Enzyme-Linked lmmunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre­ coated with a mouse monoclonal anti-human MMP-2 antibody. The Detection Antibody is a biotinylated rabbit polyclonal anti-human MMP-2 antibody. This kit is specifically designed to accurately measure MMP-2, including Pro MMP-2, Mature MMP-2, and Mature MMP-2/TIMP-2 complex. This kit has been analytically validated to assess MMP-2 levels in cell culture supernatant, serum, and heparin plasma samples with ready­ to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-human Total MMP-2 Pre-coated 96-well Strip Microplate
  • Human Total MMP-2 Detection Antibody
  • Human Total MMP-2 Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
43.8 pg/mL ± 15.9 pg/mL for serum and heparin plasma samples, 193.5 pg/mL ± 138.5 pg/mL for cell culture supernatant samples.
Standard Range
156 - 10,000 pg/mL for serum and heparin plasma samples, 625 - 40,000 pg/mL for cell culture supernatant samples.
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Gene ID
4313 View all products for this Gene ID
UniProt
View information about MMP-2 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 12.22.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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