LEAF™ Purified anti-human Lymphotoxin beta receptor (LT-βR) Antibody

Pricing & Availability
Clone
31G4D8 (See other available formats)
Other Names
Lymphotoxin beta receptor, Tumor necrosis factor receptor superfamily member 3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
31G4D8_LEAF_030308
Human peripheral blood monocytes stained with LEAF™ purified 31G4D8, followed by biotinylated anti-mouse IgG and Sav-PE
  • 31G4D8_LEAF_030308
    Human peripheral blood monocytes stained with LEAF™ purified 31G4D8, followed by biotinylated anti-mouse IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
322004 500 µg 296€
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Description

LT-βR, also known as lymphotoxin beta receptor and tumor necrosis factor receptor superfamily member 3 is a 47 kD transmembrane protein containing 3 TNF receptor domains. The LT-βR is a receptor for the heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT). LT-βR is highly expressed in the lung, liver, and kidney and moderately expressed expressed in the heart and testes. LT-βR is weakly expressed in the brain, thymus, spleen, and lymph nodes. The cytoplasmic tail of LT-βR interacts with TRAF3, TRAF4, and TRAF5 as well as the hepatitis C virus core protein. Ligand binding to LT-βR has been shown to induce apoptosis (via TRAF3 and TRAF5) and play a role in the development of lymphoid organs. The 31G4D8 antibody has been shown to be useful for flow cytometry, Western blot, immunoprepicitation and and ELISA (detection antibody). In addition, the 31G4D8 antibody is an agonistic antibody for the LT-βR capable of inducing apoptosis (with IFN-γ treatment).

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LT-βR Fc protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
WB, IP, Apop - Reported in the literature

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, ELISA detection1, induction of apoptosis2. The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 322003/322004).

Application References

(PubMed link indicates BioLegend citation)
  1. Developer's communication
  2. Chen M-C, et al. 2003. J. Biol. Chem. 278:16073.
RRID
AB_2297250 (BioLegend Cat. No. 322004)

Antigen Details

Structure
Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD
Distribution

Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes.

Function
Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs
Interaction
Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein
Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Crowe PD, et al. 1994 Science 264:707.
Gene ID
4055 View all products for this Gene ID
UniProt
View information about LT-beta R on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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