LEAF™ Purified anti-human IFN-β Antibody

Pricing & Availability
Clone
IFNb/A1 (See other available formats)
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
IFNbA1_LEAF_IFNbeta_Antibody_1_WB_101716
Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN-β (50 ng, lane 2) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with LEAF™ purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • IFNbA1_LEAF_IFNbeta_Antibody_1_WB_101716
    Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN-β (50 ng, lane 2) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with LEAF™ purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
514003 50 µg 120€
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514004 200 µg 240€
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This product is scheduled for discontinuation as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition. The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price. Please use the availability checker to determine current availability, or contact technical service for further inquiries.
Description

The IFNb/A1 clone recognizes natural human IFN-β and recombinants β1a and β1b.It is capable of neutralizing IFN-β.

Product Details
Technical data sheet

Product Details

Reactivity
Human, epitope: Hu IFN-β site I, core peptide amino acid residues 32-56, AB loop (AB2, AB3)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HuIFN-β1b
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
ELISA, Neut - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-β bioactivity (Cat. No. 514003).

Additional reported applications (for the relevant formats) include: ELISA1.

Application References

(PubMed link indicates BioLegend citation)
  1. Redlich PN and Grossberg SE, et al. 1989. J. Immunol. 143:1887. (ELISA, Neut)
  2. Redlich PN, et al. 1991. P. Natl. Acad. Sci. USA 88:4040.
  3. Runkel L, et al. 2001. IFN & Cytokine Res. 21:931.
Product Citations
  1. Su S 2018. Cell. 175:442. PubMed
Publication Library
RRID
AB_2122764 (BioLegend Cat. No. 514003)
AB_2248803 (BioLegend Cat. No. 514004)

Antigen Details

Structure
Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
Bioactivity
Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
Cell Sources
Fibroblasts, epithelial cells
Cell Targets
Broad, includes NK cells, T suppressor cells, activated monocytes
Receptors
IFNα/βR (IFNAR-1, IFNAR-2)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U, 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
3456 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 10.17.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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