ELISA MAX™ Deluxe Set Human TNF-α

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Product Citations
human tnf-a_121609
  • human tnf-a_121609
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430204 5 plates 244€
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430215 10 Plates 469€
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430216 20 Plates 751€
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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA MAX™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA MAX™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human TNF-α ELISA MAX™ Capture Antibody (200X)
  • Human TNF-α ELISA MAX™ Detection Antibody (200X)
  • Human TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Subramanya S, et al. 2009. J. Virol. PubMed
  2. Schyth BD, et al. 2012. Nucleic Acids Res. 40:463. PubMed.
Product Citations
  1. Sánchez NC, et al. 2020. DNA Cell Biol. 39:484. PubMed
  2. Gill PS, et al. 2022. Front Immunol. 13:872652. PubMed
  3. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  4. Singh N, et al. 2018. PLoS One. 13:e0199817. PubMed
  5. Singhal J, et al. 2022. Front Cell Infect Microbiol. 12:878136. PubMed
  6. Wang H, et al. 2021. J Immunother Cancer. 9:. PubMed
  7. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  8. Subramanya S, et al. 2010. J Virol. 84:2490. PubMed
  9. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  10. Asakura M, et al. 2016. Sci Rep. 6:35633. PubMed
  11. Choi J, et al. 2018. Nucleic Acids Res. 46:1912. PubMed
  12. Pap N, et al. 2021. Food Chem Toxicol. 153:112284. PubMed
  13. Singh T, et al. 2018. J Immunol. 200:2727. PubMed
  14. McIntyre B, et al. 2015. Innate Immunity. 21: 504 - 511. PubMed
  15. Kamdee K, et al. 2021. Biomed Rep. 15:78. PubMed
  16. XS R, et al. 2015. Diabetes. 64 90. PubMed
  17. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  18. Fang W, et al. 2021. Mol Med Rep. 23:. PubMed
  19. Chabanon RM, et al. 2019. J Clin Invest. 129:1211. PubMed
  20. Lewis A, et al. 2019. Front Immunol. 10:2321. PubMed
  21. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  22. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  23. Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 - 10630. PubMed
  24. Schyth B, et al. 2012. Nucleic Acids Res. 40:4653. PubMed
  25. Wurster S, et al. 2017. Virulence. 8:1708. PubMed
  26. Dold MN, et al. 2022. Apoptosis. 27:112. PubMed
  27. Dasari C, et al. 2017. Tumour Biol. 39:1010428317698380. PubMed
  28. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  29. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  30. CarriÓn M, et al. 2016. J Leukoc Biol. 100(6):1385-1393. PubMed
  31. Girsang E, et al. 2021. PeerJ. 9:e11419. PubMed
  32. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
  33. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
2 pg/ml
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
7124 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 5    Revision Date: 04.04.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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