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- Regulatory Status
- RUO
- Other Names
- Secreted and transmembrane protein 1, Protein K-12, K12, K12 protein, SECTM1
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- Product Citations
- publications
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When recombinant human SECTM1 is immobilized at 0.5 μg/mL, biotinylated recombinant human CD7 binds in a dose-dependent manner with ED50 range of 0.6 – 3.6 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding. -
Human SECTM1 was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with biotinylated recombinant human CD7. The ED50 for this effect is 0.6 – 3.6 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 798004 | 25 µg | 150€ | ||||
| 798006 | 100 µg | 337€ | ||||
Human Secreted and transmembrane protein 1 (SECTM1), also called Protein K-12, is a type I transmembrane glycoprotein that exists as an approximately 27 kDa transmembrane and a 20 kDa soluble form. The N-terminus of SECTM1 contains two Ig-like domains and one N-linked glycosylation motif. SECTM1 is expressed on thymic epithelial, fibroblast cells, neutrophils, and also highly expressed in many cancer cells, including melanoma, breast cancer and leukemia cells, but not in peripheral lymphocytes. The expression of SECTM1 can be up-regulated by IFN-γ in many cell types. SECTM1 is located adjacent to the human CD7 gene on chromosome 17. The soluble form SECTM1 is a ligand for CD7. The SECTM1‒CD7 interaction significantly increases monocyte migration by activation of the PI3K pathway. SECTM1 is a costimulatory ligand for human CD4 and CD8 T cell proliferation and cytokine production in a CD7-dependant manner. SECTM1 also synergizes with anti-CD28 to increase the production of IL-2. Human SECTM1 protein enhances expression of NK cell activation markers, such as CD54, CD69, and CD25. Targeting to SECTM1 may potentially be used for preventing an allogeneic T cell response, autoimmune diseases, and IFN-γ related diseases.
Product DetailsProduct Details
- Source
- Human SECTM1, amino acid (Gln29-Gly145) (Accession: # Q8WVN6.2), with a linker GSSR and C-terminal human IgG1 (Pro100-Lys330), followed by 6-His tag, was expressed in CHO cells.
- Molecular Mass
- The 358 amino acid recombinant protein has a predicted molecular mass of approximately 39.87 kD. The DTT-reduced and non-reduced protein shall migrate at 50-60 kD and 80-100 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human SECTM1 is immobilized at 5 μg/mL, biotinylated human CD7 binds in a dose-dependent manner with ED50 range of 0.6 – 3.6 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
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Thymic epithelial cells and thymic fibroblasts, neutrophils, leukocytes, granulocytes, breast cancer cells, prostate cancer cells, and leukemia cells
- Function
- Costimulatory ligand for T cell proliferation, increases monocyte migration, enhances expression of human NK cell activation markers, such as CD54, CD69, and CD25
- Interaction
- hSECTM1 bound at high levels to both human T and NK cells
- Ligand/Receptor
- CD7
- Bioactivity
- Biotinylated recombinant human CD7 binds to immobilized recombinant human SECTM1 in a dose-dependent manner
- Cell Type
- Epithelial cells, Fibroblasts, Macrophages, Neutrophils, T cells
- Biology Area
- Cancer Biomarkers, Cardiovascular Biology, Cell Proliferation and Viability, Costimulatory Molecules, Immunology
- Antigen References
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- Lam GK, et al. 2005. J Clin Immunol. 25:41-9.
- Lyman SD, et al. 2000. J Biol Chem. 275:3431-7.
- Slentz-Kesler KA, et al. 1998. Genomics. 47:327-40.
- Stillwell R. and Bierer BE. 2001. Immunologic research. 24:31-52.
- Wang T, et al. 2014. J Invest Dermatol. 134:1108-1118.
- Wang T, et al. 2012. J Leukoc Biol. 91:449-59.
- Gene ID
- 6398 View all products for this Gene ID
- UniProt
- View information about SECTM1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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