ELISA MAX™ Deluxe Set Human Free SCF

Pricing & Availability
Other Names
KITLG, KIT ligand, SF, MGF, FPH2, KL-1, Kitl, SHEP7, KL1
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Free_SCF_Human_ELISA_MAX_Deluxe_122915
  • Free_SCF_Human_ELISA_MAX_Deluxe_122915
Cat # Size Price Quantity Save
442504 5 plates 236€
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number, but will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Human SCF (stem cell factor) and its receptor c-Kit (CD117) play important roles in hematopoiesis, spermatogenesis, melanogenesis, and mast cell development. c-Kit exists in both membrane bound and soluble forms. Both forms can bind SCF. SCF/c-Kit interaction in mast cells results in mast cell degranulation with release of mediators, such as histamine and inflammatory cytokines and chemokines. The soluble form of c-Kit consists of the extracellular ligand binding domain which can block soluble from SCF binding to membrane bound c-Kit and act as a natural antagonist. It has also been shown to mobilize hematopoietic stem cells from bone marrow.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience, and performance of a high-end, commmercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing cost effectiveness, value, and ease of use.

The ELISA Max™ Set Deluxe is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumathoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human SCF ELISA MAX™ Capture Antibody (200X)
  • Human SCF ELISA MAX™ Detection Antibody (200X)
  • Human SCF Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
4.0 pg/mL
Standard Range
7.8 - 500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Soaline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCL, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20. BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (acid solution e.g. 2N H2SO4) BioLegend Cat. No. 423001
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent Paper

Antigen Details

Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
4254 View all products for this Gene ID
UniProt
View information about SCF on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe sets no longer come with plates, but Coating Buffer and Assay Diluent (for blocking and dilutions) are included in the ELISA MAX™ Deluxe Sets. Plates can be ordered separately (Cat. No. 423501), and instructions on coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 04.03.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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