HRP Avidin

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Other Names
Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP
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Product Citations
publications
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405103 1 ml 88€
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Description

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Product Details
Technical data sheet

Product Details

Formulation
Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation
Avidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
ELISPOT, IHC, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Application References

(PubMed link indicates BioLegend citation)
  1. Qiu LQ, et al. 2012. J Immunol. 188:5150. PubMed
  2. McLaughlin HP, et al. 2013. J Med Microbiol. 62:185. PubMed
  3. Chan WL, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  4. Macauley MS, et al. 2014. J Biol Chem. 289:351489. PubMed
  5. Roncolato EC, et al. 2015. Infect Immun. 83:713. PubMed
Product Citations
  1. Qiu L, et al. 2012. J Immunol. 188:5150. PubMed
  2. Narayanasamy A, et al. 2011. J Proteomics. 74:2948. PubMed
  3. McLaughlin H, et al. 2013. J Med Microbiol. 62:185. PubMed
  4. Chan W, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  5. Macauley M, et al. 2014. J Biol Chem. 289:35149. PubMed
  6. Roncolato E, et al. 2015. Infect Immun . 83:713. PubMed
  7. Tada R, et al. 2015. PLoS One. 10: e0139785. PubMed
  8. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  9. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
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Go To Top Version: 2    Revision Date: 11.19.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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