HRP anti-human β2-microglobulin

Pricing & Availability
Clone
2M2 (See other available formats)
Other Names
β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
280303 50 µg 160€
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus, Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human β2-microglobulin
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

ELISA Detection - Quality tested
FC - Validated

Recommended Usage

Dilute 18 µl of HRP anti-human β2-microglobulin antibody in 12 ml of assay dilution buffer. This is sufficient for one 96 well plate.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed
  2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed
  3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
RRID
AB_2617014 (BioLegend Cat. No. 280303)

Antigen Details

Structure
Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Distribution
Leukocytes, platelets, endothelial cells, epithelial cells
Function
Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand/Receptor
CD3/TCR complex, CD8
Cell Type
Leukocytes, Platelets, Endothelial cells, Epithelial cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13.
2. Williams DB, et al. 1989. J. Immunol. 142:2796.
3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533.
4. Williams A, et al. 2002. Tissue Antigens 59:3.

Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11.15.2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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