HMB-45 Monoclonal Antibody, Purified

Pricing & Availability
HMB-45 (See other available formats)
Mouse IgG1, κ
Cat # Size Price Quantity Avail. Save
911502 6 mL 177€
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911501 1 mL 354€
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This antibody is effective in immunohistochemistry (IHC).

The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.

Concentrated Format:
Cat. No. 911501 (Formerly: SIG-3116-1000) [1 mL]

Prediluted Format:
Cat. No. 911502 (Formerly: SIG-3116-26) [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)

Product Details
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody HMB-45 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of HMB-45. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Phosphate-buffered solution with BSA + 0.1% NaN3.
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:20 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)

Positive tissue (human): Melanoma

Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature

AB_2565113 (BioLegend Cat. No. 911502)
AB_2565112 (BioLegend Cat. No. 911501)


  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.

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Go To Top Version: 5    Revision Date: 08.21.2020

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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