Go-ChIP-Grade™ Purified anti-STAT6 Antibody

Pricing & Availability
Clone
16G12A08 (See other available formats)
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
16G12A08_ChIP_STAT6_antibody_1_010818
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT6 (clone 16G12A08), or B) equal amount of purified Mouse Ig1, κ isotype control antibody (Cat. No. 401401) or C) competitor’s ChIP-grade purified anti-STAT6 antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 16G12A08_ChIP_STAT6_antibody_1_010818
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT6 (clone 16G12A08), or B) equal amount of purified Mouse Ig1, κ isotype control antibody (Cat. No. 401401) or C) competitor’s ChIP-grade purified anti-STAT6 antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 16G12A08_PURE_STAT6_Antibody_2_WB_011618
    Total lysates (15 µg protein) from HeLa (lane1), NTERA-2 (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-STAT6 Antibody, clone 16G12A08 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-STAT6 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 16G12A08_PURE_STAT6_Antibody_3_IF_060816
    Jurkat cells were stained with purified anti-STAT6 (clone 16G12A08) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 16G12A08_PURE_STAT6_Antibody_4_IP_060816
    Immunoprecipitation of STAT6 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-STAT6 antibody (clone 16G12A08, lane 4). Western blot was performed using anti-STAT6 antibody (clone 16G12A08). The astrostar indicates immunoglobulin heavy chain and light chain.
Cat # Size Price Quantity Avail. Save
657905 25 µg 60€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
657906 100 µg 188€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Y641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, transclocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT6 recombinant protein (678-847 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC, IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728493 (BioLegend Cat. No. 657905)
AB_2728494 (BioLegend Cat. No. 657906)

Antigen Details

Structure
847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization. The C-terminal LXXLL motif is required for interaction with NCOA1.
Distribution

Cytoplasm. Translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene. 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene. 20:6660.
6. Takeda K, et al. 1996. Nature 380:627.

Gene ID
6778 View all products for this Gene ID 20852 View all products for this Gene ID
UniProt
View information about STAT6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 01.12.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account