Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) Antibody

Pricing & Availability
Clone
13A3-1 (See other available formats)
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_13A3-1_GoChIP_STAT3_Phospho_Antibody_071717
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone 13A3-1), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (#400101), or C) competitor’s ChIP-grade Purified anti-STAT3 Phospho (Tyr705) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 1_13A3-1_GoChIP_STAT3_Phospho_Antibody_071717
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone 13A3-1), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (#400101), or C) competitor’s ChIP-grade Purified anti-STAT3 Phospho (Tyr705) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 2_13A3-1_GoChIP_STAT3_Phospho_Antibody_1_071717
    Total cell lysate from PC3 (lane 1, 15 µg), untreated HepG2 (lane 2, 15 µg), HepG2 treated with 10 ng/ml of IL-6 for 15 minutes (lane 3, 15 µg) and HeLa (lane 4, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1 µg/ml Purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 3_13A3-1_GoChIP_STAT3_Phospho_Antibody_2_071717
    15 µg of total cell lysate from untreated NIH3T3 (lane 1) and NIH3T3 treated with 100 ng/ml of IL-6 for 30 minutes (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
651015 25 µg 60€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
651016 100 µg 188€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated modified synthetic peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 by volume. For Western blotting the suggested working dilution is 0.5 µg per ml antibody dilution buffer for each mini-gel. 

It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
 

RRID
AB_2715801 (BioLegend Cat. No. 651015)
AB_2715802 (BioLegend Cat. No. 651016)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous.

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources
Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Regulation
The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 07.20.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account