Go-ChIP-Grade™ Purified anti-STAT2 Antibody

Pricing & Availability
Clone
W15088A (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W15088A_GoChIP_STAT2_Antibody_1_052118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15088A_GoChIP_STAT2_Antibody_1_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15088A_GoChIP_STAT2_Antibody_2_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (Clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • W15088A_PURE_STAT2_Antibody_110816.jpg
    Total cell lysates (15 µg protein) from Jurkat, 293T and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-STAT2 antibody (clone W15088A) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (Cat No. 643807) was used as a loading control (lower).
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693303 25 µg 84€
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693304 100 µg 199€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. No. 693302 is available as an alternative.
Description

STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT1, p48, and CREB binding protein.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant protein of human STAT2 (671-851 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.

Application Notes

Clone W15088A does not cross-react with mouse (in-house tested).

Application References

(PubMed link indicates BioLegend citation)
  1. Bluyssen HA, Levy DE. 1997. J. Biol. Chem. 272:4600.
  2. Qureshi SA, et al. 1996. Mol. Cell. Biol. 16:288.
RRID
AB_2721599 (BioLegend Cat. No. 693303)
AB_2721600 (BioLegend Cat. No. 693304)

Antigen Details

Structure
STAT family, contains coiled coil and SH2 domain, 113 kD.
Distribution

Translocates into nucleus after phosphorylation. Expressed in skin, thymus.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT.
Interaction
STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Bluyssen HA, Levy DE. 1997. J. Biol. Chem. 272:4600.
  2. Qureshi SA, et al. 1996. Mol. Cell. Biol. 16:288.
Gene ID
6773 View all products for this Gene ID
UniProt
View information about STAT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11.30.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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