Go-ChIP-Grade™ Protein G Enzymatic Kit

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Other Names
Chromatin Immunoprecipitation Assay, ChIP assay
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Go-ChIP-Grade_ProteinG_Enzymatic_Kit_102617
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from HeLa cells with either A) 1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II Antibody (clone 8WG16, Cat. No. 664911 & 664912), B) equal amount of Purified Mouse IgG2a Isotype Control Antibody (Cat. No. 400201), or C) competitor’s ChIP-grade Purified anti-RNA Polymerase II Antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • Go-ChIP-Grade_ProteinG_Enzymatic_Kit_102617
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from HeLa cells with either A) 1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II Antibody (clone 8WG16, Cat. No. 664911 & 664912), B) equal amount of Purified Mouse IgG2a Isotype Control Antibody (Cat. No. 400201), or C) competitor’s ChIP-grade Purified anti-RNA Polymerase II Antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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699904 6 columns 156€
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Description

Go-ChIP-Grade™ Protein G Enzymatic Kit provides a sensitive, reliable, and efficient method for enzymatic Chromatin IP. The kit is complete with all reagents and buffers necessary for ChIP assay and it revolutionizes the use of spin columns for ChIP assay. Each column contains a disc of an inert, porous polymer to which protein G has been covalently attached. During the assay, the target chromatin/antibody complex is retained by the disc. Flushing with three wash buffers and an elution step are all that is required to obtain the DNA fragments of interest. 

Go-ChIP-Grade™ Protein G Enzymatic Kit is preferable to other methods because:

  • Go-ChIP-Grade™ Protein G Enzymatic Kit columns eliminate the needs for beads.
  • Go-ChIP-Grade™ Protein G Enzymatic Kit columns contain a proprietary porous polymer disc to which protein G has been covalently anchored. Its pores have inner surfaces specifically designed to maximize chromatin capture efficiency.
  • The disc base material is chemically inert, reducing incidences of non-specific binding.
  • All chromatin capture takes place inside the disc, therefore there is no dependence on risky pipette separation procedures.
  • The standard protocol does not require pre-blocking and pre-wash steps.
  • Flushing away unbound chromatin and other unwanted material is easily achieved by using washing steps that are fast and less prone to error.
     
Product Details
Technical data sheet

Kit Contents

Kit Contents
Component Size/Volume Storage Temperature  (°C) 
Chromatrap® Protein G Spin Columns 6 columns 4
Column Conditioning Buffer 15mL 4
Wash Buffer 1 12mL 4
Wash Buffer 2 12mL 4
Wash Buffer 3 12mL 4
1.3M Glycine 5mL 4
Enzymatic Lysis Buffer 2.5mL 4
Digestion Buffer 2.5mL 4
ChIP-Seq Enzymatic Stop Solution 50uL 4
ChIP-Seq Elution Buffer 1mL 4
Hypotonic Buffer 2.5mL 4
5M NaCl 125uL 4
1M NaHCO3 125uL 4
Shearing Cocktail 25uL -20
Protease Inhibitor Cocktail (PIC) 25uL -20
Proteinase K Stop Solution 25uL -20
Proteinase K 25uL -20

Go-ChIP-Grade™ anti-RNA Polymerase II Antibody; clone 8WG16 (Positive control antibody)

50uL 4

Mouse IgG2a, k Isotype Control Antibody

50uL 4
Forward primer for GAPDH (human promoter region) 25uL -20
Reverse primer for GAPDH (human promoter region) 25uL -20
1.5 ml Collection Tubes 12 RT
DNA Purification Columns 6 RT
DNA Binding Buffer 3.75mL RT
DNA Wash Buffer 3.75mL RT
DNA Elution Buffer 500uL RT

 

For more information on individual components, please refer to the product manual.


Chromatrap® Technology: This patented format is unique and has been granted in the UK (Patent No. GB2482209), the US (Patent No. 9523681), China (Patent No. ZL 2011 8 0067254.X) Japan (Patent No. JP 6088434) and Australia (Patent No. AU 2011340263).

Product Details

Storage & Handling
Please refer to the product manual for component storage temperatures.
Application

ChIP - Quality tested

Application References

(PubMed link indicates BioLegend citation)
  1. Ohtsuka H, et al. 2017. Mol. Microbiol. 105:84. (ChIP)
  2. Muralidharan S, et al. 2014. J. Immunol. 193:1975. (ChIP)
  3. Giebel NL, et al. 2016. Drug Metab. Dispos. 44:1027. (ChIP)

Antigen Details

Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Gene ID
NA
UniProt
View information about Protein G on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.22.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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