Fixation Buffer

Pricing & Availability
Regulatory Status
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
Cat # Size Price Quantity Check Availability Save
420801 100 mL 44€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical data sheet

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.

ICFC - Quality tested

Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  2. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  3. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  4. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  5. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  6. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  7. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  8. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  9. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  10. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  11. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  12. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  13. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  14. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed
  15. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  16. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  17. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  18. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  19. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  20. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  21. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  22. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  23. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  24. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  25. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  26. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  27. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  28. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  29. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  30. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  31. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  32. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  33. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  34. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  35. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  36. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  37. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  38. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  39. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  40. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  41. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  42. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  43. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  44. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  45. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  46. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  47. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  48. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  49. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  50. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  51. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  52. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  53. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  54. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  55. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  56. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  57. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  58. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  59. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  60. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  61. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  62. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  63. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  64. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07.25.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account