FITC anti-mouse Granzyme C Antibody

Pricing & Availability
Clone
SFC1D8 (See other available formats)
Other Names
CCP2, Ctla5, Ctla-5, AI323531, B10, GzmC
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
SFC1D8_FITC_GranzymeC_Antibody_1_121217
CD3 and CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 APC and Granzyme C (clone SFC1D8) FITC (Top) or Armenian Hamster IgG FITC isotype control (Bottom). Dot plots are gated on live cells.
  • SFC1D8_FITC_GranzymeC_Antibody_1_121217
    CD3 and CD28 stimulated BALB/c splenocytes (in the presence of recombinant mouse IL-2) were stained with CD8 APC and Granzyme C (clone SFC1D8) FITC (Top) or Armenian Hamster IgG FITC isotype control (Bottom). Dot plots are gated on live cells.
  • SFC1D8_FITC_GranzymeC_Antibody_2_121217
See FITC spectral data
Cat # Size Price Quantity Avail. Save
150805 25 µg 92€
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150806 100 µg 228€
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Description

Granzyme C is a serine protease that is found in the granules of cytotoxic T lymphocytes and natural killer cells (NKs). It induces the apoptosis of target cells by the externalization of phosphatidylserine, nuclear condensation and collapse, as well as the introduction of single-stranded DNA nicks. It can also induce apoptosis via mitochondrial swelling, which results in the loss of membrane potential. Reported studies show that granzyme C can support CTL-mediated killing in the absence of granzyme A or B. Additionally, wild-type mice have delayed upregulation of granzyme C relative to granzyme B and a mutation in the granzyme B gene shows earlier upregulation of granzyme C. Further studies show that in the event that granzyme B fails, the mouse models infected with persistent viruses have delayed expression of protection provided by granzyme C.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified recombinant granzyme C in CFA and boosted in IFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 μg  per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Cai Sheng, et al. 2009. J. Immunol. 182:6287. (ICFC)
RRID
AB_2721581 (BioLegend Cat. No. 150805)
AB_2721582 (BioLegend Cat. No. 150806)

Antigen Details

Distribution

Cytotoxic T lymphocytes and natural killer (NK) cells.

Function
Induces apoptotic cell death.
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Bots M, et al. 2006. J. Cell. Sci. 119:5011.
2. Ley TJ, et al. 2003. Blood 101:3093.
3. Revell PA2005. J. Immunol. 2005. 174:2124.
4. Kelso A. 2002. Int. Immunol. 6:605.
5. Getachew Y, et al. 2008. J. Immunol. 181:7810.
6. Cai Sheng, et al. 2009. J. Immunol. 182:6287.

Gene ID
14940 View all products for this Gene ID
UniProt
View information about Granzyme C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.13.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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