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FITC anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-4 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
RM4-4
C57BL/6 mouse splenocytes stained with RM4-4 FITC
  • RM4-4
    C57BL/6 mouse splenocytes stained with RM4-4 FITC
Compare all formats See FITC spectral data
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116003 50 µg 76€
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116004 500 µg 235€
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Description

CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Mackel JJ, et al. 2022. Front Cell Infect Microbiol. 12:974175. PubMed
  2. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  3. Ibrahim M, et al. 2016. J Evid Based Complementary Altern Med. 21: 171 - 176. PubMed
  4. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  5. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  6. Jin Q, et al. 2022. Drug Des Devel Ther. 16:3297. PubMed
  7. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  8. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  9. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  10. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  11. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  12. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  13. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  14. Benton K, et al. 2009. Regul Pept. 158:68. PubMed
  15. Schubert M, et al. 2021. Cancers (Basel). 13: . PubMed
  16. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_313688 (BioLegend Cat. No. 116003)
AB_313689 (BioLegend Cat. No. 116004)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 2    Revision Date: 01.02.2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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