DRAQ5™

Pricing & Availability
Regulatory Status
RUO
Other Names
Live/Dead Staining, Dead cell exclusion dye, Cell viability dye
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Product Citations
publications
a-DRAQ5_2_FC_042414
To study cell cycle analysis, C57BL/6 mouse bone marrow cells were stained with DRAQ5™ and acquired on a 633 nm laser-equipped flow cytometer. The above histogram shows DRAQ5™ detected with the 715 nm filter (PE/Cy7 channel).
  • a-DRAQ5_2_FC_042414
    To study cell cycle analysis, C57BL/6 mouse bone marrow cells were stained with DRAQ5™ and acquired on a 633 nm laser-equipped flow cytometer. The above histogram shows DRAQ5™ detected with the 715 nm filter (PE/Cy7 channel).
  • b-DRAQ5_1_ICFC_012721
    C57BL/6 mouse bone marrow cells were stained with CD45 FITC and DRAQ5™ (1:1000). Cells were acquired on a 633nm laser-equipped flow cytometer.
  • c-DRAQ5_ICC_012721
    HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 µg/ml of EGFR (clone AY13) Brilliant Violet 421™ (blue) for three hours at room temperature. Nuclei were counterstained with 1:250 dilution of DRAQ5 (red). The image was captured with a 20 x objective.
  • d-DRAQ5_IHC-F_012721
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/mL of CD3 (clone 17A2) Alexa Fluor® 647 (green), and 2.5 µg/mL of B220 (clone RA3-6B2) Brilliant Violet 421™ (blue) overnight at 4°C. Nuclei were counterstained with 1:250 of DRAQ5 (red). The image was captured by 10X objective.
  • e-DRAQ5_IHC-P_012721
    Human paraffin-embedded placenta tissue slice was prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0 M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD39 (clone A1) Brilliant Violet 421™ (blue) at 4°C overnight. Nuclei were counter stained with 1:250 dilution of DRAQ5™ (red). The image was captured with a 10X objective.
See DRAQ5™ spectral data
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424101 50 µL 148,00€
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Description

DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells. It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters. DRAQ5™ can also be used in cell imaging and is a good replacement for DAPI, as it does not get excited by UV or Violet lasers and is sub-optimally excited by the 488 nm laser. DRAQ5™ can be combined with FITC, PE, and other UV or violet excitable dyes for multi-color analysis.

Product Details
Technical data sheet

Product Details

Preparation
DRAQ5™ is supplied at 50 µL per vial (5 mM).
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
DRAQ5™ should be stored between 2°C and 8°C upon receipt.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P - Verified

Recommended Usage

DRAQ5™ is a trademark of Biostatus Limited.

Application Notes

DRAQ5™ is a cell-permeant DNA binding anthraquinone dye that intercalates between A-T bases of dsDNA. Due to its cell permeability, this dye is useful for assessing DNA content and cell cycle but is not suitable to be used as a viability dye. In microscopy applications, DRAQ5™ is also useful as a nuclear counterstain for both live and fixed specimens. It is a far-red emitting dye with optimal excitation/ emission peaks of 633nm/ 695nm, respectively, (when intercalated into DNA) that can be detected in the Alexa Fluor® 647, APC and Alexa Fluor® 700 channels. For use in cell cycle analysis, the optimal concentration must be determined for each cell type. Also, due to the wide emission spectrum and the brightness of DRAQ5™ off the 633nm red laser and the dim emission intensity of the APC-Cy7 or APC-Fire™ 750 fluorophores, flow cytometric panels should be optimized to determine that including DRAQ5™ in a panel is compatible with the use of the APC-Cy7 or APC-Fire™ 750 channel as well.

Protocol for DNA staining using DRAQ5™:

1. Perform surface staining following protocol of choice.

2. Wash cells twice with phosphate buffered saline (PBS). Sodium azide interferes with DRAQ5™ staining, thus it is recommended to stain in PBS (without calcium, magnesium, or sodium azide) or culture medium.

3. Dilute DRAQ5™ to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. Good results in flow cytometry and microscopy applications have been observed using a 1:200 to 1:1000 dilution.

4. Incubate at room temperature, preventing exposure to light, for 10-15 minutes. Subsequent washing can help make the cell cycle profile more distinct but is not absolutely necessary since DRAQ5™ is a fluorogenic reagent.

5. Analyze cells on a cytometer equipped with the 633 red laser. If performing cell cycle analysis, detection in the Alexa 700 channel with a 680LP or 715LP filter might help with resolution of the emission peaks.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Webb A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3418-y. PubMed
  2. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  3. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  4. Li Y, et al. 2020. Theranostics. 10:11376. PubMed
  5. Sándor N, et al. 2016. PLoS One. 11: 0163120. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Smith PJ, et al. 1999. J. Immunol. Methods. 229:131.
2. Smith PJ, et al. 2000. Cytometry. 40:280.
3. Yuan CM, et al. 2004. Cytometry B. Clin. Cytom. 58:47.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 01.27.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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